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KMID : 1377020190160060605
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Tissue Engineering and Regenerative Medicine
2019 Volume.16 No. 6 p.605 ~ p.614
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Evaluation of Polycaprolactone-Associated Human Nasal Chondrocytes as a Therapeutic Agent for Cartilage Repair
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Kim Do-Hyun
Lim Mi-Hyun
Jeun Jung-Ho
Park Sun-Hwa
Lee Weon-Sun
Park Sang-Hi
Kwon Mi-Yeon
Hwang Se-Hwan
Kim Sung-Won
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Abstract
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Background: In this study, we manufactured a complex of human nasal septal cartilage (hNC) with polycaprolactone (PCL) for transplantation into cartilaginous skeletal defects and evaluated their characteristics.
Methods: Nasal septum tissue was obtained from five patients aged ≥?20 years who were undergoing septoplasty. hNCs were isolated and subcultured for three passages in vitro. To formulate the cell?PCL complex, we used type I collagen as an adhesive between chondrocyte and PCL. Immunofluorescence staining, cell viability and growth in the hNC?PCL complex, and mycoplasma contamination were assessed.
Results: hNCs in PCL showed viability ≥?70% and remained at these levels for 9 h of incubation at 4 °C. Immunostaining of the hNC?PCL complex also showed high expression levels of chondrocyte-specific protein, COL2A1, SOX9, and aggrecan during 24 h of clinically applicable conditions.
Conclusion: The hNC?PCL complex may be a valuable therapeutic agent for implantation into injured cartilage tissue, and can be used clinically to repair cartilaginous skeletal defects. From a clinical perspective, it is important to set the short duration of the implantation process to achieve effective functional implantation.
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KEYWORD
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Chondrocyte, Collagen, Human nasal septum, Tissue engineering, Cartilage
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